WeightSnap Tools / Reconstitution calculator

Peptide Reconstitution Calculator

Enter your vial size, how much bacteriostatic water you add, and your dose. Get the concentration, the volume to draw, and the insulin syringe units. Works for any peptide. Free, instant, nothing to install.

50 units
on a U-100 insulin syringe
0.5 mL
volume per injection
5 mg/mL
concentration
4 doses
per vial at this dose

How the math works

Reconstitution is one division and one multiplication. The water you add sets the concentration, and the concentration converts your dose into a syringe reading.

Concentration = vial (mg) ÷ water (mL)
Volume per injection = dose (mg) ÷ concentration (mg/mL)
Syringe units (U-100) = volume (mL) × 100

Worked example: a 10 mg vial with 2 mL of bacteriostatic water gives 5 mg/mL. A 2.5 mg dose is 2.5 ÷ 5 = 0.5 mL. On a U-100 insulin syringe, 0.5 mL reads as 50 units. The same vial with 1 mL of water would be 10 mg/mL, and the same dose would read 25 units.

More water never changes how much peptide is in the vial, only how dilute it is. People usually pick a water volume that makes their dose land on a round syringe number.

Quick reference: units at common concentrations

Dose2.5 mg/mL5 mg/mL10 mg/mL
0.25 mg10 units5 units2.5 units
0.5 mg20 units10 units5 units
1 mg40 units20 units10 units
2.5 mg100 units50 units25 units
5 mg200 units100 units50 units

A U-100 insulin syringe holds 100 units (1 mL). Entries over 100 units will not fit in a single 1 mL syringe.

Frequently asked questions

What does reconstituting a peptide mean?

Peptides usually ship as a freeze-dried powder. Reconstitution is adding a liquid, most often bacteriostatic water, so the powder dissolves and can be measured with a syringe. The amount of water sets the concentration; it never changes the total amount of peptide in the vial.

How much bacteriostatic water should I add?

Any amount works mathematically. More water means a more dilute mix and a larger volume per dose. Most people choose a volume that puts their dose on an easy syringe number, like the 10 mg + 2 mL example above. For anything specific to your prescription, ask your pharmacist or provider.

How many units is my dose on an insulin syringe?

It depends entirely on your concentration. Divide your dose in mg by the concentration in mg/mL to get mL, then multiply by 100 for U-100 units. The calculator above does this for you.

Does this work for semaglutide, tirzepatide, and retatrutide?

Yes. The math is identical for anything measured in milligrams and dissolved in a known volume, from compounded GLP-1 medications to research peptides like BPC-157 or ipamorelin.

Is this medical advice?

No. This is an arithmetic tool: it converts the numbers you enter. It does not recommend doses or products. Always confirm anything affecting your health with your pharmacist or healthcare provider.

Doing this math every time you mix a new vial? The WeightSnap app stores your vial and concentration, shows the units for each entry automatically, and tracks how much is left in the vial so you know when to reorder. It also answers the bigger question: whether the protocol is actually working. Mixing a multi-peptide blend like Wolverine or KLOW? Use the blend calculator for per-component amounts. Want a drug-specific version with its own chart? Try the retatrutide, tirzepatide, or semaglutide calculators. For the personal side of all this, the founder's dosing protocol and before & after are on the blog, and the research hub has real-world data from 14,252 patient reports.

WeightSnap is a tracking tool, not medical advice. This calculator performs arithmetic on the numbers you enter and makes no recommendations. Always work with your healthcare provider.